Supporting information for Exploitable length correlations in peptide nanofibres
نویسندگان
چکیده
Peptide synthesis: peptides were assembled on an automatic microwave peptide synthesizer, Liberty1 (CEM Corp.), using Fmoc-Gln(Trt)-Wang resin and standard solid phase Fmoc/tBu protocols and HBTU/DIPEA as coupling reagents. Following synthesis completion the peptides were cleaved from the resin by TFA:TIS:H2O – 95:2.5:2.5%. Peptides were purified by semi-preparative RP-HPLC on a JASCO HPLC system (model PU-980; Tokyo, Japan) and their purity and identity were confirmed by analytical HPLC and MALDI-ToF mass spectrometry (Bruker Daltonics) with 2,5-dihydroxybenzoic acid as a matrix. MS [M+H] + : T2 – m/z 3067.7 (calc.), 3069.6 (found); T3 – m/z 4574.7 (calc.), 4573.3 (found); T4 – m/z 6084.2 (calc.), 6083.9 (found); T3(-) – m/z 4575.6 (calc.), 4575.7 (found).
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